Bidirectional immobilization of affinity-tagged cytochrome c on electrode surfaces.
نویسندگان
چکیده
Here, we report a new strategy for the directed bivalent immobilization of cyt c on or between gold electrodes. C-terminal modification with cys- or his-tag did not affect the functional integrity of the protein. In combination with electrostatic protein binding, these tags enable a bifunctional immobilization between two electrodes or alternatively one electrode and interacting enzymes.
منابع مشابه
Specific and Reversible Immobilization of Proteins Tagged to the Affinity Polypeptide C-LytA on Functionalized Graphite Electrodes
We have developed a general method for the specific and reversible immobilization of proteins fused to the choline-binding module C-LytA on functionalized graphite electrodes. Graphite electrode surfaces were modified by diazonium chemistry to introduce carboxylic groups that were subsequently used to anchor mixed self-assembled monolayers consisting of N,N-diethylethylenediamine groups, acting...
متن کاملPreparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c
Objective(s): The present work deals with the preparation of nanobioconjugates based on the immobilization of cytochrome c (cyt c) on functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect of the nanosupport and the immobilization procedure on the biochemical and structural characteristics of the immobilized protein was investigated. Methods: </strong...
متن کاملThe spatial and sequential immobilisation of cytochrome c at adjacent electrodes.
Two adjacent electrode surfaces were modified in a sequential manner with self-assembled thiol layers from the same solution using conditions (aqueous buffer at neutral pH) suitable for applications with proteins. A faradaic response was obtained from the redox protein, cytochrome c, independently immobilised at each surface.
متن کاملCharacterization of protein-attached conducting polymer monolayer.
Cytochrome c (cyt c)-immobilized monolayers and multiple monolayers of a conducting polymer [poly(terthiophene-3-carboxylic acid) polymer (poly-TTCA)] were prepared, where the monolayer of monomer precursor was fabricated with the Langmuir-Blogett technique. Covalent immobilization of cyt c was achieved by the formation of an amide bond between the carboxylic groups of the conducting polymer an...
متن کاملElectron transfer and ligand binding to cytochrome c' immobilized on self-assembled monolayers.
We have successfully immobilized Allochromatium vinosum cytochrome c' on carboxylic acid-terminated thiol monolayers on gold and have investigated its electron-transfer and ligand binding properties. Immobilization could only be achieved for pH's ranging from 3.5 to 5.5, reflecting the fact that the protein is only sufficiently positively charged below pH 5.5 (pI = 4.9). Upon immobilization, th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 46 29 شماره
صفحات -
تاریخ انتشار 2010